The hierarchical packing of euchromatin domains can be described as multiplicative cascades

The genome is packed into the cell nucleus in form of chromatin. Biochemical approaches have revealed that chromatin within domains, which group larger and so forth. Such hierarchical packing equally visible super-resolution microscopy images large-scale organization. While previous work has suggested partitioned distinct domains via microphase separation, it unclear how these organize this packing. A particular challenge to find an image analysis approach fully incorporates such packing, hypothetical governing mechanisms euchromatin can be compared against results analysis. Here, we obtain 3D STED from pluripotent zebrafish embryos labeled with improved DNA fluorescence stains, demonstrate described as multiplicative cascades. Multiplicative cascades are established theoretical concept describe placement ever-smaller structures bigger structures. Importantly, generate artificial data by applying a single rule again again, specified using only four parameters. show typical patterns organization reflected values Specifically, pinpoint required mimic microphase-separated state euchromatin. We suggest also applied other types cascade parameters could serve test quantities assess whether separation or models accurately reproduce